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Activity Gradients
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2016
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Endeavor
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Project Title: |
Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? |
Project Status: |
Submitted |
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Principal Investigator: |
Carol Arnosti, UNC_CH |
Project Institution: |
UNC_CH |
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Project ID: |
103796 |
Version #: |
1 |
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Date Submitted: |
2/9/2013 7:11:00 PM |
Created By: |
Carol Arnosti |
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Date Last Modified: |
7/15/2013 4:12:00 PM |
URI Serial #: |
None |
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Funding Agencies: |
NSF/OCE/CO - 1332881 - Funded |
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Summary of Field Work: |
We will investigate spatial and depth-related gradients in the abilities of heterotrophic microbial communities to initiate carbon cycling via activities of their extracellular enzymes. We will carry out incubation experiments with water collected during the cruise.
Both transect cruises are intended to resolve spatial and depth-related differences in carbon cycling processes, for which we will be collecting water with Niskin/CTD casts and processing samples for measurement of microbial community composition, activities and particulate and dissolved organic matter composition in depth profiles through the water column. The transects will ideally be conducted during late spring (May-June) to coincide with times of higher primary productivity. |
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Summary of Facility Requirements: |
We need Niskin rosette and CTD, a radioisotope van, a -80 freezer, and two refrigerators. If a cold van can fit also aboard ship, it would be very helpful. |
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Summary of other requirements and comments: |
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Type of Request: |
Primary Ship Use |
Request Status: |
Submitted |
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Request ID: |
1006052 |
Created By: |
Carol Arnosti |
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Date Last Modified: |
2/9/2013 7:11:00 PM |
Date Submitted: |
2/9/2013 7:11:00 PM |
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Year: |
2016
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Ship/Facility: |
Endeavor
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Optimum Start Date:
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5/1/2016 |
Dates to Avoid: |
We would like to catch a period of rising primary productivity, so late spring/early summer is most suitable. |
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Earliest Start Date: |
4/20/2016 |
Multi-Ship Op: |
No |
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Latest Start Date: |
6/20/2016 |
Other Ship(s): |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
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14 |
1 |
1 |
1 |
17 |
Repeating Cruise?
(within same year) |
No |
Interval: |
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# of Cruises: |
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Description of Repeating cruise requirements: |
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Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
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Short Description of Op Area
for use in schedules: |
E/SE Cp Hattera |
| Description of Op Area: |
The transect should go SE Cape Hatteras, off the Blake Ridge, and then head due east into the Sargasso Sea. |
| Op Area Size/Dia.: |
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Lat/Long |
Marsden Grid |
Navy Op Area |
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Beginning
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34° 22.8' N
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76° 24' W
map
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Ending
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34° 22.8' N
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76° 24' W
map
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Show Degrees Minutes |
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Foreign Clearance Required? |
No |
Coastal States:
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 Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
| Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
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Beaufort, NC, USA |
None |
Beaufort, NC, USA |
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops |
Close to transect start. |
Important Info on Working in Foreign Ports
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Chief Scientist: |
Carol Arnosti, UNC_CH |
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# in Science Party |
15 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
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Explanation of Science Party Requirements and Technician Requirements |
We will have specific teams dealing with each type of analysis/sample/experiment that we will be conducting. Separate groups will be in the rad van, cold van, and labs. A marine tech is needed to help us with CTD/Niskin work. |
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 Dynamic Positioning | ADCP | Multibeam | Seismic |
| Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
| SCUBA Diving |  Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
| 0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling. |
We will be measuring bacterial productivity (leucine incorporation) and therefore need a rad van.
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Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems. |
The rad van is required for incubation of samples to measure bacterial protein production. If Endeavor has sufficient deck space, the cold van would be very helpful for processing and incubation of sub-thermocline samples. |
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