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Deep-sea vent micro
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2013
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Atlantis
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Project Title: |
Dimensions: Collaborative Research: An Integrated Approach to Study Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents |
Project Status: |
Submitted |
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Principal Investigator: |
Stefan M. Sievert, WHOI |
Project Institution: |
WHOI |
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Project ID: |
102921 |
Version #: |
1 |
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Date Submitted: |
3/24/2011 9:33:00 PM |
Created By: |
Stefan M. Sievert |
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Date Last Modified: |
12/23/2011 10:32:00 AM |
URI Serial #: |
None |
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Funding Agencies: |
NSF/BIO/DEB - 1136727 - Funded |
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Summary of Field Work: |
We propose one cruise that will take place within the first year of the project (~February 2013). The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454-pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since our last visit to the site (May 2010). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. In situ carbon fixation will be determined using a modified Incubating Productivity System (IPS) at the two focus sites, as well as selected additional sites. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms. We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses. |
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Summary of Facility Requirements: |
large ship like R/V Atlantis, HOV (e.g. Alvin) or ROV (e.g. Jason) |
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Summary of other requirements and comments: |
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Type of Request: |
Primary Ship Use |
Request Status: |
Submitted |
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Request ID: |
1004532 |
Created By: |
Stefan M. Sievert |
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Date Last Modified: |
3/24/2011 9:33:00 PM |
Date Submitted: |
3/24/2011 9:33:00 PM |
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Year: |
2013
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Ship/Facility: |
Atlantis
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Optimum Start Date:
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2/1/2013 |
Dates to Avoid: |
July-November because of weather (storms) |
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Earliest Start Date: |
7/1/2013 |
Multi-Ship Op: |
No |
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Latest Start Date: |
5/7/2013 |
Other Ship(s): |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
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16 |
1 |
1 |
4 |
22 |
Repeating Cruise?
(within same year) |
No |
Interval: |
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# of Cruises: |
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Description of Repeating cruise requirements: |
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Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
we are proposing to use HOV Alvin or ROV Jason. For Alvin we would need Atlantis, for Jason similar ship |
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Short Description of Op Area
for use in schedules: |
9º50' N EPR |
| Description of Op Area: |
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| Op Area Size/Dia.: |
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Lat/Long |
Marsden Grid |
Navy Op Area |
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Beginning
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9.833333° N
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104.3° W
map
9° 49.99998' N
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104° 18' W
map
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Ending
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9.833333° N
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104.3° W
map
9° 49.99998' N
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104° 18' W
map
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Show Degrees Minutes |
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Foreign Clearance Required? |
No |
Coastal States:
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 Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
| Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
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Manzanillo, Mexico |
None |
Manzanillo, Mexico |
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops |
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Important Info on Working in Foreign Ports
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Chief Scientist: |
Stefan M. Sievert, WHOI |
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# in Science Party |
23 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
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Explanation of Science Party Requirements and Technician Requirements |
This is an interdisciplinary and multinational project |
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 Dynamic Positioning |  ADCP | Multibeam | Seismic |
| Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
| SCUBA Diving | Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
| 0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling. |
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Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems. |
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