Ship Request

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  Deep-sea vent micro  -  2013  -  Atlantis  
  Project Information  
Project Title: Dimensions: Collaborative Research: An Integrated Approach to Study Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents Project Status: Submitted
Principal Investigator: Stefan M. Sievert, WHOI Project Institution: WHOI
Project ID: 102921 Version #: 1
Date Submitted: 3/24/2011 9:33:00 PM Created By: Stefan M. Sievert
Date Last Modified: 12/23/2011 10:32:00 AM URI Serial #: None
Funding Agencies: NSF/BIO/DEB - 1136727 - Funded
Summary of Field Work: We propose one cruise that will take place within the first year of the project (~February 2013). The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454-pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since our last visit to the site (May 2010). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. In situ carbon fixation will be determined using a modified Incubating Productivity System (IPS) at the two focus sites, as well as selected additional sites. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms. We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.
Summary of Facility Requirements: large ship like R/V Atlantis, HOV (e.g. Alvin) or ROV (e.g. Jason)
Summary of other requirements and comments:  
Ship Request Identification
Type of Request: Primary Ship Use Request Status: Submitted
Request ID: 1004532 Created By: Stefan M. Sievert
Date Last Modified: 3/24/2011 9:33:00 PM Date Submitted: 3/24/2011 9:33:00 PM
Requested Ship, Operating Days and Dates
Year: 2013 Ship/Facility: Atlantis
Optimum Start Date: 2/1/2013 Dates to Avoid: July-November because of weather (storms)
Earliest Start Date: 7/1/2013 Multi-Ship Op: No
Latest Start Date: 5/7/2013 Other Ship(s):

Operating Days Needed: Science Days Mob Days De-Mob Days Estimated Transit Days Total Days
16 1 1 4 22
Repeating Cruise?
(within same year)
No Interval:   # of Cruises:  

Description of Repeating cruise requirements:
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations:
we are proposing to use HOV Alvin or ROV Jason. For Alvin we would need Atlantis, for Jason similar ship
Work Area for Cruise
Short Description of Op Area
for use in schedules:
9º50' N EPR
Description of Op Area:
Op Area Size/Dia.:  
  Lat/Long Marsden Grid Navy Op Area
9.833333° N / 104.3° W map
11 map
NP13 map
9.833333° N / 104.3° W map
11 map
NP13 map
  Show Degrees Minutes    
Foreign Clearance and Permitting Requirements
Foreign Clearance Required? No Coastal States:
 Important Info on Foreign Research Clearances  

Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
No If yes, have you applied for the necessary permits through your export control office? No
 Questions about ITAR/EAR regulations?

Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.)
Port Calls
Requested Start Port Intermediate Port(s) Requested End Port
Manzanillo, Mexico None Manzanillo, Mexico
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops

 Important Info on Working in Foreign Ports

Science Party
Chief Scientist: Stefan M. Sievert, WHOI
# in Science Party 23 # of different science teams 1 # Marine Technicians to be
provided by ship operator:
(include in science party total)
Explanation of Science Party Requirements and Technician Requirements This is an interdisciplinary and multinational project
Instrumentation Requirements That Impact Scheduling Decisions
Selected Dynamic PositioningUnselected ADCPSelected MultibeamUnselected Seismic
Unselected Dredging/Coring/Large Dia. Trawl WireUnselected Stern A-frameUnselected Fiber Optic (.681)Unselected 0.680 Coax Wire
Unselected SCUBA DivingUnselected Radioisotope use - briefly describeUnselected NO Radioisotope use/Natural level workUnselected Other Operator Provided Inst. - Describe
0 PI-Provided Vans - briefly describe Unselected MOCNESS  
Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling.

Major Ancillary Facilities (that require coordination of schedules with ship schedule)
Unselected Helicopter Ops (USCG)Unselected Twin OtterUnselected Unmanned Aerial Systems (UAS) 
Autonomous Underwater Vehicle (AUV)
Unselected Other AUVUnselected Sentry  
Coring Facility
Unselected Jumbo Piston CoringUnselected Large Gravity Core Unselected MC800 multicorer w/ MISO camera/telemetryUnselected OSU Coring Facility (MARSSAM)
Unselected Other Large Coring FacilityUnselected WHOI Long Core  
Human Occupied Vehicle (HOV)
Selected AlvinUnselected Clelia (HBOI)Unselected JSL I & II (HBOI)Unselected Other HOV
Other Facility
Unselected MISO Facility - deep-sea imagingUnselected Other FacilityUnselected Potential Fields Pool Equipment 
Remotely Operated Vehicle (ROV)
Selected JasonUnselected Other ROV  
Seismic Facility
Unselected Ocean Bottom Seismograph Instrument Center (OBSIC)Unselected Ocean Bottom Seismograph Instrument Pool (OBSIP)Unselected Ocean-Bottom Seismometer Program (UTIG)Unselected Other Seismic/OBS Facility
Unselected PASSCALUnselected Portable MCS groupUnselected Portable MCS/SCS groupUnselected U.S. Geological Survey Ocean Bottom Seismometer Facility (USGS at WHOI)
Towed Underwater Vehicle
Unselected ARGO IIUnselected Hawaii MR1 (HMRG)Unselected IMI12 (HMRG)Unselected IMI120 (HMRG - formerly DSL 120A)
Unselected IMI30 (HMRG)Unselected Other Towed Underwater VehicleUnselected Towfish 
UNOLS Van Pool
Unselected AUV Lab Van #1Unselected Clean Lab VanUnselected Cold Lab VanUnselected General Purpose Lab Van
Unselected Radioisotope Lab VanUnselected Wet Lab Van  
UNOLS Winch Pool
Unselected Mooring SpoolerUnselected Portable WinchUnselected Turn Table 
Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems.

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