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Antarctic chemoauto
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2018
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Laurence M. Gould
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Project Title: |
Collaborative Research: Chemoautotrophy in Antarctic bacterioplankton communities supported by the oxidation of urea-derived nitrogen |
Project Status: |
Submitted |
Principal Investigator: |
James Hollibaugh, Georgia |
Project Institution: |
Georgia |
Project ID: |
105270 |
Version #: |
2 |
Date Submitted: |
5/3/2016 7:57:00 PM |
Created By: |
James Hollibaugh |
Date Last Modified: |
6/8/2017 4:56:00 PM |
URI Serial #: |
None |
Funding Agencies: |
NSF/OPP/ANT - 1643466 - Funded |
Summary of Field Work: |
Water sampling at established stations in the Palmer LTER domain off the West Antarctic Peninsula. Samples would be collected using the ship's rosette sampler/CTD at approximately 3 depths per station, to be chosen in consultation with the cruise's Chief Scientist, but nominally at 10, 50 and 400 m depth over the continental shelf and from 1500 m off the shelf. Water samples (2 L from each depth) will be processed in the dry lab (as long as a sink is available). Processing includes filtration for RNA and DNA samples, dissolved inorganic nitrogen and urea analysis (urea and ammonium samples to be processed onboard). Filtered samples (filters and water samples) will be frozen at -80C for subsequent processing ashore. In addition, replicate incubations (250 mL bottles) with 15N-labeled substrates will be set up and run for 48 hr, then preserved by freezing at -80. We will also measure dark NaH14CO3 incorporation to estimate total heterotrophy. |
Summary of Facility Requirements: |
dry lab, access to CTD rosette, clean lab for nutrient analysis (shared lab van OK), isotope van (14C, shared van OK) |
Summary of other requirements and comments: |
none |
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Type of Request: |
Ancillary |
Request Status: |
Submitted |
Request ID: |
1008504 |
Created By: |
James Hollibaugh |
Date Last Modified: |
5/12/2016 1:27:00 PM |
Date Submitted: |
5/3/2016 7:57:00 PM |
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Year: |
2018
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Ship/Facility: |
Laurence M. Gould
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Optimum Start Date:
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1/1/2018 |
Dates to Avoid: |
N/A |
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Earliest Start Date: |
12/28/2017 |
Multi-Ship Op: |
No |
Latest Start Date: |
1/5/2018 |
Other Ship(s): |
N/A |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
30 |
2 |
2 |
10 |
44 |
Repeating Cruise?
(within same year) |
No |
Interval: |
N/A |
# of Cruises: |
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Description of Repeating cruise requirements: |
N/A |
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
N/A |
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Short Description of Op Area
for use in schedules: |
PAL-LTER Grid |
Description of Op Area: |
Continental shelf and slope west of the Antarctic Peninsula sampled by PAL-LTER |
Op Area Size/Dia.: |
3000 |
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Lat/Long |
Marsden Grid |
Navy Op Area |
Beginning
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Ending
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Foreign Clearance Required? |
Yes |
Coastal States:
Chile |
Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or description of any other special permitting requirements (e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
N/A |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
Rada Punta Arenas, Chile |
Palmer Station, Antarctica, Palmer Station, Antarctica |
Rada Punta Arenas, Chile |
Explanation/justification for requested ports and dates of intermediate stops or to list additional port stops |
Punta Arenas is the nearest airport, the Gould stops at Palmer to offload/pick up supplies and personnel |
Important Info on Working in Foreign Ports
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Chief Scientist: |
James Hollibaugh, Georgia |
# in Science Party |
2 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
Explanation of Science Party Requirements and Technician Requirements |
Work planned involves a lot of filtration, isotope (15N and 14C) experiments, and incubations lasting 24 hr, and to measure nitrite and ammonium concentrations at sea. |
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Dynamic Positioning | ADCP | Multibeam | Seismic |
Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
SCUBA Diving | Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability requirements that could affect choice of ship in scheduling. |
NaH14CO3 used to measure chemoautotrophic production
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Explain Major Ancillary Facilities Requirements and list description and provider for "other" systems. |
Radioisotope lab van needed for chemoautotrophy experiments |
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