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NFIXPACIFIC
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2019
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Sally Ride
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Project Title: |
Investigating Diazotrophy in tropical and subtropical Pacific Ocean |
Project Status: |
Submitted |
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Principal Investigator: |
Jonathan Zehr, UCSC |
Project Institution: |
UCSC |
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Project ID: |
106319 |
Version #: |
6 |
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Date Submitted: |
11/30/2018 4:56:00 PM |
Created By: |
Esther Wing Kwan Mak |
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Date Last Modified: |
2/19/2019 6:42:00 PM |
URI Serial #: |
None |
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Funding Agencies: |
INST/UCSD/SIO - NONE - Funded |
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Summary of Field Work: |
We propose to study the diazotroph community from Guam to San Diego on R/V Sally Ride, An area that is theoretically ideal for biological nitrogen fixation yet highly under sampled. We will focus on the community composition of nitrogen-fixing bacteria, nitrogen release through grazing experiments, as well as visualising and quantifying the nitrogen fixation rates of an abundant heterotrophic diazotroph Gamma A.
We will collect DNA, RNA, and bulk N fixation rate samples for surveying, CARD/gene-FISH samples followed by nano secondary ion mass spectrometer (SIMS) for measuring cell-specific N fixation rates. We will also carry out dilution experiments to measure the selective grazing pressure on different diazotrophs. |
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Summary of Facility Requirements: |
We will need a CTD rosette, sufficient deck space for incubation experiments and access to a fume hood or outdoor bench space. Our water budget is 6L for 6 depths (~15, 25, 75, 100, 125, 150m, flexible) at each station and 9 L and 18 L for nitrogen fixation and grazing incubations, respectively. |
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Summary of other requirements and comments: |
We are highly flexible to the ship track, station locations and water budget. The cruise plan can be adjusted based on other groups on board. |
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Type of Request: |
Ancillary |
Request Status: |
Submitted |
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Request ID: |
1010183 |
Created By: |
Esther Wing Kwan Mak |
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Date Last Modified: |
2/19/2019 6:42:00 PM |
Date Submitted: |
11/30/2018 4:56:00 PM |
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Year: |
2019
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Ship/Facility: |
Sally Ride
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Optimum Start Date:
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11/1/2019 |
Dates to Avoid: |
We have no dates to avoid, and we don’t have any requirement on the start date and end date, and thus can accommodate to other groups on board. |
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Earliest Start Date: |
10/1/2019 |
Multi-Ship Op: |
No |
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Latest Start Date: |
12/31/2019 |
Other Ship(s): |
N/A |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
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18 |
1 |
1 |
0 |
20 |
Repeating Cruise?
(within same year) |
No |
Interval: |
N/A |
# of Cruises: |
1 |
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Description of Repeating cruise requirements: |
N/A |
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
We are highly flexible to the timing and number of days on R/V Sally Ride. Our aim is to collect spatially high-resolution samples across the Guam to San Diego. |
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Short Description of Op Area
for use in schedules: |
Guam-SD |
| Description of Op Area: |
We propose to join the UC Ship Funds programme and require ship time for R/V Sally Ride in their transect from Guam to San Diego |
| Op Area Size/Dia.: |
5371.35 |
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Lat/Long |
Marsden Grid |
Navy Op Area |
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Beginning
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13.4443° N
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144.7937° W
map
13° 26.658' N
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144° 47.622' W
map
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Ending
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32.8959° N
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117.2424° W
map
32° 53.754' N
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117° 14.544' W
map
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Show Degrees Minutes |
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Foreign Clearance Required? |
No |
Coastal States:
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 Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
| Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
N/A |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
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Apra Harbor, Guam |
None, |
San Diego, CA, USA |
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops |
We propose to join the UC Ship Funds programme and require ship time for R/V Sally Ride in their transect from Guam to San Diego. We don't have a requirement on a specific port that R/V Sally Ride decided to use. |
Important Info on Working in Foreign Ports
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Chief Scientist: |
Kendra Turk-Kubo, UCSC |
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# in Science Party |
2 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
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Explanation of Science Party Requirements and Technician Requirements |
We request 2 of us, Katie Harding and Esther Mak to be on board, due to the human power needed to do sampling and set up incubations. We do not have a specific technician requirement. |
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 Dynamic Positioning | ADCP | Multibeam | Seismic |
| Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
| SCUBA Diving | Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
| 0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling. |
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Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems. |
N/A |
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