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Cocco-Mix
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2018
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Endeavor
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Project Title: |
Coccolithophore Mixotrophy |
Project Status: |
Submitted |
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Principal Investigator: |
William M. Balch, Bigelow |
Project Institution: |
Bigelow |
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Project ID: |
105219 |
Version #: |
2 |
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Date Submitted: |
2/15/2016 1:44:00 PM |
Created By: |
William M. Balch |
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Date Last Modified: |
7/15/2016 12:27:00 PM |
URI Serial #: |
None |
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Funding Agencies: |
NSF/OCE/BIO - 1635748 - Funded |
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Summary of Field Work: |
A 12-d cruise is planned, crossing the Gulf of Maine on the line between Portland, ME towards Yarmouth, NS then turning SSE and steaming to Bermuda. We will be sampling water for coccolithophores and performing isotope incubations with various dissolved organic carbon compounds and fluorescently labeled bacteria. We will use a flow cytometer to separate the coccolithophores from the rest of the phytoplankton and examine their ability to take up take up this DOC as well as bacterial-sized particles in a mixotrophic fashion. |
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Summary of Facility Requirements: |
We will need a CTD/rosette system plus sufficient space on deck for a radioisotope van plus the Bigelow Laboratory flow-cytometer van (8'x8'x20' container). |
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Summary of other requirements and comments: |
We will plan on visiting 7 stations enroute to Bermuda, occupying each for about 1d. The total steaming time would be slightly less than 5d. |
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Type of Request: |
Primary Ship Use |
Request Status: |
Submitted |
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Request ID: |
1008360 |
Created By: |
William M. Balch |
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Date Last Modified: |
7/15/2016 12:27:00 PM |
Date Submitted: |
2/15/2016 1:44:00 PM |
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Year: |
2018
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Ship/Facility: |
Endeavor
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Optimum Start Date:
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6/15/2018 |
Dates to Avoid: |
N/A |
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Earliest Start Date: |
6/1/2018 |
Multi-Ship Op: |
No |
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Latest Start Date: |
7/1/2018 |
Other Ship(s): |
N/A |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
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12 |
2 |
2 |
1 |
17 |
Repeating Cruise?
(within same year) |
No |
Interval: |
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# of Cruises: |
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Description of Repeating cruise requirements: |
N/A |
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
Coccolithophore abundance peaks in the NW Atlantic near the summer solstice. This is the reason that we are focusing on the period near the beginning of summer. |
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Short Description of Op Area
for use in schedules: |
NW Atlantic |
| Description of Op Area: |
We'll begin in the Gulf of Maine, traveling from Portland, ME towards S. Nova Scotia (traveling into Canadian waters, then head SSE to Bermuda |
| Op Area Size/Dia.: |
1137 nautical miles |
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Lat/Long |
Marsden Grid |
Navy Op Area |
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Beginning
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43.667° N
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70.2667° W
map
43° 40.02' N
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70° 16.002' W
map
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Ending
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32.379° N
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64.678° W
map
32° 22.74' N
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64° 40.68' W
map
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Show Degrees Minutes |
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Foreign Clearance Required? |
Yes |
Coastal States:
Bermuda, Canada |
 Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
| Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
N/A |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
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Portland, ME, USA |
None, , None, |
St. George's, Bermuda |
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops |
I am requesting to begin the cruise in Portland, ME due to its proximity to Bigelow Laboratory for Ocean Sciences, in East Boothbay, Maine. We have loaded R/V Endeavor there for past cruises, and there was sufficient water depth at various piers. St. George, Bermuda is the best spot of offload, unless the ship will head straight back to Rhode Island, in which case we'd kindly request to leave our gear on the ship and offload it as soon as the ship arrives in Narragansett. |
Important Info on Working in Foreign Ports
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Chief Scientist: |
William M. Balch, Bigelow |
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# in Science Party |
8 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
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Explanation of Science Party Requirements and Technician Requirements |
The science party will need personnel for helping to deploy CTD/water sample (2), do water filtration(2), run FlowCAM (1) work in the rad van (2), run the flow cytometer (1). I am requesting a marine tech for assistance with running the CTD/Rosette and supervising deck operations. |
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 Dynamic Positioning | ADCP | Multibeam | Seismic |
| Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
| SCUBA Diving |  Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
| 0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling. |
The work we propose will be using the unstable radio-isotope, 14C (associated with bicarbonate plus various organic compounds that will be determined in the first year of lab work). This will require a radioisotope van for a) manipulating samples and b) housing a scintillation counter. At each station, ten water samples (five in low light and five in complete darkness) will be incubated in 1L tissue culture bottles in an environmental incubator. Post-incubation, these will be centrifuged to concentrate the coccolithophores. This concentrated sample will be used with the flow cytometer (in separate Bigelow Laboratory container...also certified for use with isotopes) to sort coccolithophores for isotope measurements.
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Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems. |
Radioisotope van usage described above. |
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