Ship Request

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  Cocco-Mix  -  2018  -  Endeavor  
  Project Information  
Project Title: Coccolithophore Mixotrophy Project Status: Submitted
Principal Investigator: William M. Balch, Bigelow Project Institution: Bigelow
Project ID: 105219 Version #: 2
Date Submitted: 2/15/2016 1:44:00 PM Created By: William M. Balch
Date Last Modified: 7/15/2016 12:27:00 PM URI Serial #: None
Funding Agencies: NSF/OCE/BIO - 1635748 - Funded
Summary of Field Work: A 12-d cruise is planned, crossing the Gulf of Maine on the line between Portland, ME towards Yarmouth, NS then turning SSE and steaming to Bermuda. We will be sampling water for coccolithophores and performing isotope incubations with various dissolved organic carbon compounds and fluorescently labeled bacteria. We will use a flow cytometer to separate the coccolithophores from the rest of the phytoplankton and examine their ability to take up take up this DOC as well as bacterial-sized particles in a mixotrophic fashion.
Summary of Facility Requirements: We will need a CTD/rosette system plus sufficient space on deck for a radioisotope van plus the Bigelow Laboratory flow-cytometer van (8'x8'x20' container).
Summary of other requirements and comments: We will plan on visiting 7 stations enroute to Bermuda, occupying each for about 1d. The total steaming time would be slightly less than 5d.
Ship Request Identification
Type of Request: Primary Ship Use Request Status: Submitted
Request ID: 1008360 Created By: William M. Balch
Date Last Modified: 7/15/2016 12:27:00 PM Date Submitted: 2/15/2016 1:44:00 PM
Requested Ship, Operating Days and Dates
Year: 2018 Ship/Facility: Endeavor
Optimum Start Date: 6/15/2018 Dates to Avoid: N/A
Earliest Start Date: 6/1/2018 Multi-Ship Op: No
Latest Start Date: 7/1/2018 Other Ship(s): N/A

Operating Days Needed: Science Days Mob Days De-Mob Days Estimated Transit Days Total Days
12 2 2 1 17
Repeating Cruise?
(within same year)
No Interval:   # of Cruises:  

Description of Repeating cruise requirements: N/A
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations:
Coccolithophore abundance peaks in the NW Atlantic near the summer solstice. This is the reason that we are focusing on the period near the beginning of summer.
Work Area for Cruise
Short Description of Op Area
for use in schedules:
NW Atlantic
Description of Op Area: We'll begin in the Gulf of Maine, traveling from Portland, ME towards S. Nova Scotia (traveling into Canadian waters, then head SSE to Bermuda
Op Area Size/Dia.: 1137 nautical miles
  Lat/Long Marsden Grid Navy Op Area
43.667° N / 70.2667° W map
152 map
NA06 map
32.379° N / 64.678° W map
115 map
NA06 map
  Show Degrees Minutes    
Foreign Clearance and Permitting Requirements
Foreign Clearance Required? Yes Coastal States:

Bermuda, Canada
 Important Info on Foreign Research Clearances  

Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
No If yes, have you applied for the necessary permits through your export control office? No
 Questions about ITAR/EAR regulations?

Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.)
Port Calls
Requested Start Port Intermediate Port(s) Requested End Port
Portland, ME, USA None, , None, St. George's, Bermuda
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops
I am requesting to begin the cruise in Portland, ME due to its proximity to Bigelow Laboratory for Ocean Sciences, in East Boothbay, Maine. We have loaded R/V Endeavor there for past cruises, and there was sufficient water depth at various piers. St. George, Bermuda is the best spot of offload, unless the ship will head straight back to Rhode Island, in which case we'd kindly request to leave our gear on the ship and offload it as soon as the ship arrives in Narragansett.

 Important Info on Working in Foreign Ports

Science Party
Chief Scientist: William M. Balch, Bigelow
# in Science Party 8 # of different science teams 1 # Marine Technicians to be
provided by ship operator:
(include in science party total)
Explanation of Science Party Requirements and Technician Requirements The science party will need personnel for helping to deploy CTD/water sample (2), do water filtration(2), run FlowCAM (1) work in the rad van (2), run the flow cytometer (1). I am requesting a marine tech for assistance with running the CTD/Rosette and supervising deck operations.
Instrumentation Requirements That Impact Scheduling Decisions
Unselected Dynamic PositioningUnselected ADCPUnselected MultibeamUnselected Seismic
Unselected Dredging/Coring/Large Dia. Trawl WireUnselected Stern A-frameUnselected Fiber Optic (.681)Unselected 0.680 Coax Wire
Unselected SCUBA DivingSelected Radioisotope use - briefly describeUnselected NO Radioisotope use/Natural level workUnselected Other Operator Provided Inst. - Describe
0 PI-Provided Vans - briefly describe Unselected MOCNESS  
Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling.

The work we propose will be using the unstable radio-isotope, 14C (associated with bicarbonate plus various organic compounds that will be determined in the first year of lab work). This will require a radioisotope van for a) manipulating samples and b) housing a scintillation counter. At each station, ten water samples (five in low light and five in complete darkness) will be incubated in 1L tissue culture bottles in an environmental incubator. Post-incubation, these will be centrifuged to concentrate the coccolithophores. This concentrated sample will be used with the flow cytometer (in separate Bigelow Laboratory container...also certified for use with isotopes) to sort coccolithophores for isotope measurements.

Major Ancillary Facilities (that require coordination of schedules with ship schedule)
Unselected Helicopter Ops (USCG)Unselected Twin OtterUnselected Unmanned Aerial Systems (UAS) 
Autonomous Underwater Vehicle (AUV)
Unselected Other AUVUnselected Sentry  
Coring Facility
Unselected Jumbo Piston CoringUnselected Large Gravity Core Unselected MC800 multicorer w/ MISO camera/telemetryUnselected OSU Coring Facility (MARSSAM)
Unselected Other Large Coring FacilityUnselected WHOI Long Core  
Human Occupied Vehicle (HOV)
Unselected AlvinUnselected Clelia (HBOI)Unselected JSL I & II (HBOI)Unselected Other HOV
Other Facility
Unselected MISO Facility - deep-sea imagingUnselected Other FacilityUnselected Potential Fields Pool Equipment 
Remotely Operated Vehicle (ROV)
Unselected JasonUnselected Other ROV  
Seismic Facility
Unselected Ocean Bottom Seismograph Instrument Center (OBSIC)Unselected Ocean Bottom Seismograph Instrument Pool (OBSIP)Unselected Ocean-Bottom Seismometer Program (UTIG)Unselected Other Seismic/OBS Facility
Unselected PASSCALUnselected Portable MCS groupUnselected Portable MCS/SCS groupUnselected U.S. Geological Survey Ocean Bottom Seismometer Facility (USGS at WHOI)
Towed Underwater Vehicle
Unselected ARGO IIUnselected Hawaii MR1 (HMRG)Unselected IMI12 (HMRG)Unselected IMI120 (HMRG - formerly DSL 120A)
Unselected IMI30 (HMRG)Unselected Other Towed Underwater VehicleUnselected Towfish 
UNOLS Van Pool
Unselected AUV Lab Van #1Unselected Clean Lab VanUnselected Cold Lab VanUnselected General Purpose Lab Van
Selected Radioisotope Lab VanUnselected Wet Lab Van  
UNOLS Winch Pool
Unselected Mooring SpoolerUnselected Portable WinchUnselected Turn Table 
Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems.
Radioisotope van usage described above.

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