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Microbial DO respir
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2021
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Pelican
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Project Title: |
CAREER: Who's sucking all the oxygen out of the room? Investigating aerobic microbial respiration dynamics in coastal hypoxia. |
Project Status: |
Submitted |
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Principal Investigator: |
Cameron Thrash, USC |
Project Institution: |
USC |
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Project ID: |
106537 |
Version #: |
1 |
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Date Submitted: |
7/18/2019 9:10:00 PM |
Created By: |
Cameron Thrash |
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Date Last Modified: |
3/12/2020 8:34:00 PM |
URI Serial #: |
None |
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Funding Agencies: |
NSF/OCE/BIO - 1945279 - Funded |
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Summary of Field Work: |
The general research plan will be to collect samples via CTD at 5 stations across the northern Gulf of Mexico shelf between the Mississippi River southwest pass and the Atchafalaya delta.
Sampling
Sampling will be conducted with a CTD rosettes for physico-chemical depth profiles of DO, temperature, and salinity. Discrete samples 20-40L will be collected from surface and bottom water for core biogeochemical measurements including size-fractionated chlorophyll-a, nitrite, nitrate, silica, soluble reactive phosphorus, ammonium, and pH. These same samples will be filtered for DOM collection and characterization. Microbiological sampling will use water (1-4 L) filtered serially through 2.7 µm GF/D (Whatman), and 0.2 µm Sterivex (Millipore) filters. All samples will be stored at -20°C until processing back at USC.
Shipboard measurements and experiments
Additional water (2 x 10 mL) will be collected and fixed with 10% formalin and stored at 4°C for cell counts shipboard using a Guava EasyCyte flow cytometer. Both whole water and 2.7 µm-filtered water will be collected for DO respiration measurements. Respiration will be measured in BOD bottles (completely filled) using optodes (PreSens). Incubations will occur in foil-darkened bottles at in-situ temperatures for 24 hours, with measurements taken every hour. Microbial metabolic activity will be assessed by incubating organisms with transcriptional and respiration-specific fluorescence dyes at in-situ temperatures for the multiple days. Post-incubation samples will be fixed and frozen for transport to USC. |
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Summary of Facility Requirements: |
We will need access to a CTD/rosette with DO, turbidity and fluorescence measurement capability, as well as 10 L niskin bottles. We will also need onboard lab space for filtration, flow cytometry measurements, and experimental setup. -20°C freezer, 4°C refrigerator, storage space for incubations, surface intake access, deck storage for a large seawater container. |
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Summary of other requirements and comments: |
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Type of Request: |
Collaborative Ship Use |
Request Status: |
Submitted |
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Request ID: |
1010533 |
Created By: |
Cameron Thrash |
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Date Last Modified: |
7/18/2019 9:10:00 PM |
Date Submitted: |
7/18/2019 9:10:00 PM |
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Year: |
2021
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Ship/Facility: |
Pelican
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Optimum Start Date:
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5/1/2021 |
Dates to Avoid: |
We want to avoid dates that move into summer to avoid overlap with the subsequent cruise for the year. |
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Earliest Start Date: |
4/21/2021 |
Multi-Ship Op: |
No |
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Latest Start Date: |
5/21/2021 |
Other Ship(s): |
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Operating Days Needed: |
Science Days |
Mob Days |
De-Mob Days |
Estimated Transit Days |
Total Days |
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5 |
1 |
1 |
1 |
8 |
Repeating Cruise?
(within same year) |
Yes |
Interval: |
biannual |
# of Cruises: |
2 |
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Description of Repeating cruise requirements: |
Cruises need to take place in mid spring, before the onset of northern Gulf of Mexico hypoxia, and during mid summer, when hypoxia is peaking. Ideally these will occur in early May and late July/early August. |
Justification/Explanation for ship choice, dates,
conflicts, number of days & multi-ship operations: |
The Pelican is the closest ship to the region that will be studied, and has all the appropriate space and instrument facilities required to perform the requested work. |
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Short Description of Op Area
for use in schedules: |
East GOM shelf |
| Description of Op Area: |
The operations area will consist of the C to F transect region from the annual shelfwide hypoxia cruises led by Dr. Nancy Rabalais. This consists of the area from the Mississippi River southwest pass to the Atchafalaya delta. |
| Op Area Size/Dia.: |
150 |
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Lat/Long |
Marsden Grid |
Navy Op Area |
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Beginning
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28.8686° N
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90.4903° W
map
28° 52.116' N
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90° 29.418' W
map
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Ending
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28.7833° N
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91.6167° W
map
28° 46.998' N
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91° 37.002' W
map
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Show Degrees Minutes |
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Foreign Clearance Required? |
No |
Coastal States:
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 Important Info on Foreign Research Clearances
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Are you or any member in your science party bringing in any science equipment items which are regulated for export by the International Traffic in Arms Regulations (ITAR) and/or the Export Administration Regulations (EAR)?
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No |
If yes, have you applied for the necessary permits through your export control office?
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No |
| Questions about ITAR/EAR regulations?
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Comments about foreign clearance requirements or
description of any other special permitting requirements
(e.g., MMPA, ESA, IHA, Marine Sanctuaries, etc.) |
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Requested Start Port |
Intermediate Port(s) |
Requested End Port |
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Cocodrie, LA, USA |
None, |
Cocodrie, LA, USA |
Explanation/justification for requested
ports and dates of intermediate stops
or to list additional port stops |
This is the closest port to the sampling region. |
Important Info on Working in Foreign Ports
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Chief Scientist: |
Cameron Thrash, USC |
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# in Science Party |
2 |
# of different science teams |
1 |
# Marine Technicians to be
provided by ship operator:
(include in science party total)
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1 |
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Explanation of Science Party Requirements and Technician Requirements |
There will be one science party consists of one team of two people. A technician is required for help with CTD operations. |
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 Dynamic Positioning | ADCP | Multibeam | Seismic |
| Dredging/Coring/Large Dia. Trawl Wire | Stern A-frame | Fiber Optic (.681) | 0.680 Coax Wire |
| SCUBA Diving | Radioisotope use - briefly describe | NO Radioisotope use/Natural level work | Other Operator Provided Inst. - Describe |
| 0 PI-Provided Vans - briefly describe | MOCNESS | | |
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Explain Instrumentation or Capability
requirements that could affect choice
of ship in scheduling. |
N/A
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Explain Major Ancillary Facilities
Requirements and list description
and provider for "other" systems. |
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